Authors have no one to acknowledge.

1. Device Fabrication
<ol>
	<li>Design two photomasks, the first for patterning the surface acoustic wave (SAW) layer, and the second for the polydimethylsiloxane (PDMS) microchannel mold.
	<ol>
		<li>The first photomask has a pair of opposing interdigital transducers (IDTs)&#8212;also known as a SAW delay line&#8212;and markers for channel alignment and spatial reference during microscopy. In our standard device we have single-electrode IDTs with a finger width p = 10 &#956;m, aperture of 750 &#956;m, and 25 straight finger pairs. The resulting IDT generates SAWs with a wavelength &#955; = 4p = 40 &#956;m corresponding to an operating frequency fo = cSAW/&#955; &#8776; 100 MHz on 128&#176; YX lithium niobate (LN). Each IDT width should be above two times the width of the microchannel to reduce any misalignment effects while bonding the layers. IDT design parameters are discussed comprehensively in several books9-11. We remark that only one IDT (placed at the channel outlet) is necessary to drive the fluid into the channel in acoustic-counterflow, but patterning a full delay line assists in device testing.</li>
		<li>The second has a simple microchannel structure to be aligned along the SAW delay line, with a microchamber to form the channel inlet. In our typical devices, the channels have a width w = 300 mm and a length of 5 mm. As a general rule, the channel width should be at least 10&#955; to avoid diffraction effects during SAW propagation in the microchannel, however in our testing we found that a width of ~7&#955; would not significantly affect SAW propagation within the channel.</li>
	</ol>
	</li>
	<li>Begin with an LN wafer and cleave a 2 cm by 2 cm sample. In order to perform transmission microscopy it is necessary to use a double side polished wafer. Note that LN is a standard for its biocompatibility and the SAW polarization and high piezoelectric coupling coefficient along the major axis, however other piezoelectric materials may be used with appropriate design considerations.</li>
	<li>Clean the substrate by rinsing it in acetone, 2-propanol and drying with a nitrogen gun.</li>
	<li>Spin coat the sample with Shipley S1818 at 4,000 rpm for 1 min.</li>
	<li>Soft bake at 90 &#176;C for 1 min on a hot plate.</li>
	<li>Align the sample with the SAW layer mask using a mask aligner and expose it to UV light with a 55 mJ/cm2. Care should be taken to align the IDT direction along the major axis of the LN substrate.</li>
	<li>Rinse the sample in Microposit MF319 developer for 30 sec to remove the unexposed photoresist.</li>
	<li>Stop the development by rinsing the sample in deionized water and dry it with a nitrogen gun.</li>
	<li>Deposit a 10-nm-thick titanium adhesion layer followed by 100-nm-thick gold layer by thermal evaporation.</li>
	<li>Perform lift-off by sonicating the sample in acetone, then rinse it in 2-propanol and dry with a nitrogen gun.</li>
	<li>Silanize the device surface to make it hydrophobic in the microchannel area12.
	<ol>
		<li>Mask the microchamber area with AR-N-4340 negative tone photoresist by optical lithography according to the manufacturer&#39;s datasheet.</li>
		<li>Activate the sample surface with a 2 min oxygen plasma (Gambetti Kenologia Srl, Colibri) of 0.14 mbar pressure and 100 W power giving a bias voltage of approximately 450 V.</li>
		<li>Mix 35 ml hexadecane, 15 ml carbon tetrachloride (CCl4), and 20 &#956;l octadecyltrichlorosilane (OTS) into a beaker inside a fume hood. Place the device in the solution, and leave covered for two hours.</li>
		<li>Rinse the device with 2-propanol and dry it with a nitrogen gun.</li>
		<li>Check that the contact angle of water on the surface is above 90&#176;. If the contact angle is insufficient, clean the sample and re-perform the steps in 1.11.</li>
		<li>Remove the residual resist on the sample by rinsing in acetone, 2-propanol and drying with a nitrogen gun.</li>
	</ol>
	</li>
	<li>Mount the sample on a printed circuit board with radio frequency waveguides and standard coaxial connectors (RF-PCB), and then put acoustic absorber (First Contact polymer) on the sample edges and connect the IDT by wire bonding or using pogo connectors.</li>
	<li>A master mold of the channel layer is patterned with SU-8 onto a small piece of Silicon (Si) wafer using standard optical photolithography. SU-8 type and photolithography recipe will be dependent on the final PDMS internal channel height required.</li>
	<li>Cast PDMS on the mold
	<ol>
		<li>Mix PDMS with a curing agent at a ratio of 10:1.</li>
		<li>Centrifuge the PDMS for 2 min at 1,320 x g for degassing.</li>
		<li>Pour the PDMS gently onto the SU-8 mold in a Petri dish to a total PDMS height on the order of 1 mm. The open Petri dish can be placed in a vacuum desiccator for approximately 30 min in order to degas the PDMS further.</li>
		<li>Once degassed, cure PDMS by heating to 80 &#176;C for one hour in an oven. Note that baking time and temperature can affect the mechanical properties of PDMS.</li>
	</ol>
	</li>
	<li>Prepare the solid PDMS layer
	<ol>
		<li>Cut around the channel using a surgical blade, being careful not to damage the SU8 master, and peel it off.</li>
		<li>Replica edges are then refined and straightened using a razor blade leaving at least 2 mm clearance on lateral side of the channel and no clearance (cut right through) at the channel outlet.</li>
		<li>Punch a hole in the microchamber using a Harris Unicore puncher to form the fluid-loading inlet.</li>
	</ol>
	</li>
	<li>Bond the PDMS channel with the LN substrate by simple conformal bonding. In this way the bond will hold throughout the fluid testing stage while remaining reversible.
	<ol>
		<li>Both surfaces are cleaned prior to joining by blowing away any excess debris with compressed nitrogen air. It is critical when joining the pieces to align the channel with the major axis of the LN according to the patterned alignment marks.</li>
	</ol>
	</li>
	<li>The complete device schematic is shown in Figure 1. Store completed devices in a clean environment until use.</li>
</ol>
Note: It is important that all fabrication steps are carried out in a clean room environment to avoid contamination of the device before use.
Note: Any of the optical lithography steps may be replaced by the user preferred methods.
Note: The silanization procedure may be substituted for a preferred hydrophobic coating method13.
2. RF Device Testing
<ol>
	<li>Calibrate the network or spectrum analyzer with an open/short waveguide on your RF-PCB.</li>
	<li>Connect the SAW delay line to the ports of a spectrum analyzer and measure the scattering matrix of the device. The transmission for a pair of single-electrode transducers will resemble the absolute value of a sinc function centered at the operating frequency of the IDT. In the reflection spectrum a dip (minimum) is observed at the same frequency9-11. In our devices at 100 MHz operating frequency along the major axis typical values are -15 dB for S11 and S22 and -10 dB for S12 (without PDMS channels).</li>
</ol>
3. Microfluidics and Particle Flow Dynamics Visualization Experiment and Analysis
<ol>
	<li>Place the sample under a microscope. The specific optical setup depends on the SAW microfluidics phenomena to be observed. For example, a simple reflection microscope equipped with a 4X objective and a 30 fps video camera will be suitable to study fluid filling dynamics. To investigate more complex microparticle dynamics, it may be necessary to use a microscope equipped with a 20X objective and a 100 fps or higher video camera. It is important that both the objective and frame rate are high enough to capture any spatially and temporally important flow features.</li>
	<li>Connect the IDT in front of the channel outlet to an RF signal generator and operate it at the resonant frequency observed in the scattering matrix measurements. The typical operating power in acoustic-counterflow experiments is 20 dBm. If necessary, use a high-power UHF amplifier. Acoustic-streaming and atomization phenomena are observed without acoustic counterflow while running the device at lower power: typically acoustic-streaming recirculation begins at 0 dBm and atomization occurs above 14 dBm.</li>
	<li>Load 60 &#956;l of fluid into the microchamber with a micropipette. Fluid will passively diffuse into the microchamber. If necessary, gently push on the microchamber surface in order to favor the microchamber filling.
	<ol>
		<li>In order to visualize the flow it is necessary to add microbeads to the fluid. Note that in order to avoid particle clustering, sonicate the particle suspension prior to the experiments. To avoid particle adhesion on the substrate apply a 0 dBm signal to the device while loading.</li>
	</ol>
	</li>
	<li>Start recording the video through the microscope and increase the operating power in order to observe acoustic counterflow. Different flow schemes will be determined by input power, chip design and particle diameter.
	<ol>
		<li>In order to qualitatively capture the dynamics, fluid flow has to be recorded in proximity of the meniscus and inlet at different stages of channel filling using markers as a spatial reference.</li>
		<li>In order to perform quantitative measurement of particle dynamics by micro particle image velocimetry (&#956;PIV) 14,15 or spatial temporal image correlation spectroscopy (STICS) 16,17, fluid flow has to be recorded in the point of interest with a fixed field of view for at least 100 frames at a frame rate imposed by the particle dynamics.</li>
	</ol>
	</li>
	<li>Analyze the video with image processing software. The choice of the software to be used depends on the phenomena of interest. For example, to quantify the size distribution of atomized droplets, spatial periodicity of particle accumulation, or manual tracking of diluted particles, simple freeware image analysis software such as Fiji is suitable18; whereas in order to obtain streamlines and velocity field measurements, customized mPIV19 or STICS20 code is required. In our analysis customized STICS code is written in MATLAB, however a preferred alternative coding language may be equally acceptable.</li>
</ol>

<ol>
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M., Piazza, V., Beltram, F., Cecchini, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interaction-free,+automatic,+on-chip+fluid+routing+by+surface+acoustic+waves.">Interaction-free, automatic, on-chip fluid routing by surface acoustic waves.</a> Lab on a Chip. 12 (15), 2621-2624 (2012).</li><li>Wixforth, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acoustically+driven+planar+microfluidics.">Acoustically driven planar microfluidics.</a> Superlattices and Microstructures. 33 (5), 389-396 (2003).</li><li>Friend, J., Yeo, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscale+acoustofluidics:+Microfluidics+driven+via+acoustics+and+ultrasonics.">Microscale acoustofluidics: Microfluidics driven via acoustics and ultrasonics.</a> Reviews of Modern Physics. 83 (2), 647-64 (2011).</li><li>Tan, M. K., Yeo, L. Y., Friend, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+fluid+flow+and+mixing+induced+in+microchannels+using+surface+acoustic+waves.">Rapid fluid flow and mixing induced in microchannels using surface acoustic waves.</a> Europhysics Letters. 87, 47003 (2009).</li><li>Glass, N., Shilton, R., Chan, P., Friend, J., Yeo, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Miniaturised+Lab-on-a-Disc+(miniLOAD).">Miniaturised Lab-on-a-Disc (miniLOAD).</a> SMALL, Small. 8 (12), 1880-1880 (2012).</li><li>Madou, M. J., Kellogg, G. 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W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatiotemporal+image+correlation+spectroscopy+(STICS)+theory,+verification,+and+application+to+protein+velocity+mapping+in+living+CHO+cells.">Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells.</a> Biophysical Journal. 88 (5), 3601 (2005).</li><li>Rossow, M., Mantulin, W. 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Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploitation+of+surface+acoustic+waves+to+drive+size-dependent+microparticle+concentration+within+a+droplet.">Exploitation of surface acoustic waves to drive size-dependent microparticle concentration within a droplet.</a> Lab on a Chip. 10 (21), 2979-2985 (2010).</li><li>Muller, P. B., Barnkob, R., Jensen, M. J. H., Bruus, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+numerical+study+of+microparticle+acoustophoresis+driven+by+acoustic+radiation+forces+and+streaming-induced+drag+forces.">A numerical study of microparticle acoustophoresis driven by acoustic radiation forces and streaming-induced drag forces.</a> Lab on a Chip. 12 (22), 4617-4627 (2012).</li><li>Luo, J. K., Fu, Y. Q., Li, Y., Du, X. Y., Flewitt, A. J., Walton, A. J., Milne, W. 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Authors have nothing to disclose.

One of the greatest challenges faced by the microfluidic community is the realization of an actuation platform for truly portable point-of-care devices. Among the proposed integrated micropumps23,those based on surface acoustic waves (SAWs) are particularly attractive due to their associated capabilities in fluid mixing, atomization and particle concentration and separation4. In this paper we have demonstrated how to fabricate and operate a lab-on-chip device in which fluid is steered in a closed PD...

One of the continued challenges facing the microfluidic community is the need to have an efficient pumping mechanism that can be miniaturized for integration into truly portable micro-total-analysis systems (&#956;TAS&#39;s). Standard macroscopic pumping systems simply fail to provide the portability required for &#956;TAS&#39;s, owing to the unfavorable scaling of the volumetric flow rates as the channel size decreases down to the micron range or below. On the contrary, SAWs have gained increasing interest as fluid actuation mechanisms and appear as a promising avenue for the solution of some of these problems1,2.
SAWs were shown to provide a very efficient mechanism of energy transport into fluids3. When a SAW propagates onto a piezoelectric substrate, e.g. lithium niobate (LN), the wave will be radiated into any fluid in its path at an angle known as the Rayleigh angle &#952;R = sin&#8722;1 (cf /cs), owing to the mismatch of sound velocities in the substrate, cs, and the fluid cf. This leakage of radiation into the fluid gives rise to a pressure wave which drives acoustic streaming in the fluid. Depending on the device geometry and power applied to the device, this mechanism was shown to actuate a wide variety of on-chip processes, such as fluid mixing, particle sorting, atomization, and pumping1,4. Despite the simplicity and effectiveness of actuating microfluids with SAW, there are only a small number of SAW driven microfluidic pumping mechanisms that have been demonstrated to date. The first demonstration was the simple translation of free droplets placed in the SAW propagation path on a piezoelectric substrate3. This novel method generated much interest in using SAWs as a microfluidic actuation method, however there was still a need for fluids to be driven through enclosed channels&#8212;a more difficult task. Tan et al. demonstrated pumping within a microchannel that was laser ablated directly into the piezoelectric substrate. By geometric modification with respect to the channel and IDT dimensions, they were able to demonstrate both uniform and mixing flows5. Glass et al. recently demonstrated a method of moving fluids through microchannels and microfluidic components by combining SAW actuated rotations with centrifugal microfluidics, as a demonstration of true miniaturization of the popular Lab-on-a-CD concept6,7. However, the only fully enclosed SAW driven pumping mechanism that has been demonstrated remains to be Cecchini et al.&#39;s SAW-driven acoustic counterflow8&#8212;the focus of this video. It exploits the atomization and coalescence of a fluid to pump it through a closed channel in the direction opposing the propagation direction of the acoustic wave. This system can give rise to surprisingly complex flows within a microchannel. Moreover, depending on the device geometry, it can provide a range of flow schemes, from laminar flows to more complex regimes characterized by vortices and particle-accumulation domains. The ability to easily influence the flow characteristics within the device shows opportunities for advanced on-chip particle manipulation.
In this protocol we wish to clarify the main aspects of practical SAW-based microfluidics: device fabrication, experimental operation, and flow visualization. While we are explicitly describing these procedures for the fabrication and operation of SAW-driven acoustic counterflow devices, these sections can easily be modified for their application to a range of SAW-driven microfluidic regimes.

<table border="1">
	<tbody>
		<tr>
			<td>Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>Double side polished 128&#176; YX lithium niobate wafer</td>
			<td>Crystal Technology, LLC</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Silicon wafer</td>
			<td>Siegert Wafers</td>
			<td></td>
			<td>We use &#60;100&#62;</td>
		</tr>
		<tr>
			<td>IDT Optical lithography mask with alignment marks (positive)</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Channel Optical lithography mask (negative)</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Positive photoresist</td>
			<td>Shipley</td>
			<td>S1818</td>
			<td></td>
		</tr>
		<tr>
			<td>Positive photoresist developer</td>
			<td>Microposit</td>
			<td>MF319</td>
			<td></td>
		</tr>
		<tr>
			<td>Negative tone photoresist</td>
			<td>Allresist</td>
			<td>AR-N-4340</td>
			<td></td>
		</tr>
		<tr>
			<td>Negative tone photoresist developer</td>
			<td>Allresist</td>
			<td>AR 300-475</td>
			<td></td>
		</tr>
		<tr>
			<td>SU8 thick negative tone photoresist</td>
			<td>Microchem</td>
			<td>SU-8 2000 Series</td>
			<td></td>
		</tr>
		<tr>
			<td>SU8 thick negative tone photoresist developer</td>
			<td>Microchem</td>
			<td>SU-8 developer</td>
			<td></td>
		</tr>
		<tr>
			<td>Hexadecane</td>
			<td>Sigma-Aldrich</td>
			<td>H6703</td>
			<td></td>
		</tr>
		<tr>
			<td>Carbon tetrachloride (CCl4)</td>
			<td>Sigma-Aldrich</td>
			<td>107344</td>
			<td></td>
		</tr>
		<tr>
			<td>Octadecyltrichlorosilane (OTS)</td>
			<td>Sigma-Aldrich</td>
			<td>104817</td>
			<td></td>
		</tr>
		<tr>
			<td>Acetone CMOS grade</td>
			<td>Sigma-Aldrich</td>
			<td>40289</td>
			<td></td>
		</tr>
		<tr>
			<td>2-propanol CMOS grade</td>
			<td>Sigma-Aldrich</td>
			<td>40301</td>
			<td></td>
		</tr>
		<tr>
			<td>Titanium</td>
			<td>Any vendor</td>
			<td>99.9% purity</td>
			<td></td>
		</tr>
		<tr>
			<td>Gold</td>
			<td>Any vendor</td>
			<td>99.9% purity</td>
			<td></td>
		</tr>
		<tr>
			<td>PDMS</td>
			<td>Dow Corning</td>
			<td>Sylgard 184 silicone elastomer kit with curing agent</td>
			<td></td>
		</tr>
		<tr>
			<td>Petri dish</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>5 mm ID Harris Uni-Core multi-purpose coring tool</td>
			<td>Sigma-Aldrich</td>
			<td>Z708895</td>
			<td>Any diameter greater than 2 mm is suitable</td>
		</tr>
		<tr>
			<td>Acoustic absorber</td>
			<td>Photonic Cleaning Technologies</td>
			<td>First Contact regular kit</td>
			<td></td>
		</tr>
		<tr>
			<td>RF-PCB</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Spinner</td>
			<td>Laurell technologies corporation</td>
			<td>WS-400-6NPP</td>
			<td>Any spinner can be used</td>
		</tr>
		<tr>
			<td>UV Mask aligner</td>
			<td>Karl Suss</td>
			<td>MJB 4</td>
			<td>Any aligner can be used</td>
		</tr>
		<tr>
			<td>Thermal evaporator</td>
			<td>Kurt J. Lesker</td>
			<td>Nano 38</td>
			<td>Any thermal, e-beam evaporator or sputtering system can be used</td>
		</tr>
		<tr>
			<td>Oxygen plasma asher</td>
			<td>Gambetti Kenologia Srl</td>
			<td>Colibr&#236;</td>
			<td>Any plasma asher or RIE machine can be used</td>
		</tr>
		<tr>
			<td>Centrifuge</td>
			<td>Eppendorf</td>
			<td>5810 R</td>
			<td>Any centrifuge can be used</td>
		</tr>
		<tr>
			<td>Wire bonder</td>
			<td>Kulicke &#38; Soffa</td>
			<td>4523AD</td>
			<td>Any wire bonder can be used if the PCB is used without pogo connectors</td>
		</tr>
		<tr>
			<td>Contact Angle Meter</td>
			<td>KSV</td>
			<td>CAM 101</td>
			<td>Any contact angle meter can be used</td>
		</tr>
		<tr>
			<td>Spectrum analyzer</td>
			<td>Anristu</td>
			<td>56100A</td>
			<td>Any spectrum or network analyzer can be used</td>
		</tr>
		<tr>
			<td>RF signal generator</td>
			<td>Anristu</td>
			<td>MG3694A</td>
			<td>Any RF signal generator can be used</td>
		</tr>
		<tr>
			<td>RF high power amplifier</td>
			<td>Mini Circuits</td>
			<td>ZHL-5W-1</td>
			<td>Any RF high power amplifier can be used</td>
		</tr>
		<tr>
			<td>Microbeads suspension</td>
			<td>Sigma-Aldrich</td>
			<td>L3280</td>
			<td>Depending on the experimental purpose different suspension of different diameter and different material properties can be used</td>
		</tr>
		<tr>
			<td>Optical microscope</td>
			<td>Nikon</td>
			<td>Ti-Eclipse</td>
			<td>Any optical microscope with spatial resolution satisfying experimental purposes can be used</td>
		</tr>
		<tr>
			<td>Video camera</td>
			<td>Basler</td>
			<td>A602-f</td>
			<td>Any video camera that has enough frame rate and sensitivity satisfying experimental purposes can be used</td>
		</tr>
		<tr>
			<td>Camera acquisition software</td>
			<td>Advanced technologies</td>
			<td>Motion Box</td>
			<td>Any software enabling high and controlled frame rate acquisition can be used</td>
		</tr>
	</tbody>
</table>

fabrication, operation and flow visualization in surface-acoustic-wave-driven acoustic-counterflow microfluidics

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we present the fabrication protocol for a multilayered SAW acoustic counterflow device. The device is fabricated starting from a lithium niobate (LN) substrate onto which two interdigital transducers (IDTs) and appropriate markers are patterned. A polydimethylsiloxane (PDMS) channel cast on an SU8 master mold is finally bonded on the patterned substrate. Following the fabrication procedure, we show the techniques that allow the characterization and operation of the acoustic counterflow device in order to pump fluids through the PDMS channel grid. We finally present the procedure to visualize liquid flow in the channels. The protocol is used to show on-chip fluid pumping under different flow regimes such as laminar flow and more complicated dynamics characterized by vortices and particle accumulation domains.

Figure 2 shows representative results of device RF testing which were taken prior to bonding the LN layer to the microchannel layer: typical S11 and S12 spectra are reported in panel a) and b) respectively. The depth of the valley at central frequency in S11 spectrum is related to the efficiency of conversion of RF power in SAW mechanical power. Hence, for a fixed number of IDT finger pairs, a reduction in the valley minimum will result i...

Watch this Scientific Journal Video about Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics at JoVE.com

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

In this video we first describe fabrication and operation procedures of a surface acoustic wave (SAW) acoustic counterflow device. We then demonstrate an experimental setup that allows for both qualitative flow visualization and quantitative analysis of complex flows within the SAW pumping device.

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we ...

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16.9K Views.  Istituto Italiano di Tecnologia. In this video we first describe fabrication and operation procedures of a surface acoustic wave (SAW) acoustic counterflow device. We then demonstrate an experimental setup that allows for both qualitative flow visualization and quantitative analysis of complex flows within the SAW pumping device.