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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

12.7K Views

10:51 min

November 15th, 2013

DOI :

10.3791/51085-v

November 15th, 2013


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Keywords Reverse Yeast Two hybrid System

Rozdziały w tym wideo

0:05

Title

2:47

Construction of PXR and SRC-1 Fusions in Yeast Vectors

3:49

Yeast Two-hybrid Assays

5:26

X-gal Filter Assay

6:32

Liquid β-gal Assay

7:49

Results: X-gal Lift and β-gal Liquid Assay Interpretation

9:06

Conclusion

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