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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

11.3K Views

10:47 min

July 24th, 2017

DOI :

10.3791/56080-v

July 24th, 2017


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Yeast one Hybrid

Rozdziały w tym wideo

0:05

Title

0:40

Preparation of Competent Cells

2:40

Co-transformation of Cells by Heat Shock

4:39

Transferring Cells onto New Plates

5:25

Preparing Culture for Measuring β-galactosidase Activity

8:14

Measuring β-galactosidase Activity

9:07

Results: Y1.5H Assay Detects Heteromeric Protein-DNA Interactions

9:50

Conclusion

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