JoVE Logo

Zaloguj się

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

14.5K Views

15:12 min

May 10th, 2014

DOI :

10.3791/51447-v

May 10th, 2014


Transkrypcja

Przeglądaj więcej filmów

Keywords Cystic Fibrosis

Rozdziały w tym wideo

0:05

Title

1:46

Solubilization of Microsomes

3:49

Nickel-affinity Purification of CFTR

5:33

Gel Permeation Chromatography (GPC) Purification of CFTR

6:57

Reconstitution of CFTR

7:51

Measurement of ATPase Activity

9:35

Results: Isolation of Milligram Quantities of CFTR by Two Methods

14:28

Conclusion

Powiązane Filmy

article

10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

30.4K Views

article

08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

9.6K Views

article

08:24

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

3.3K Views

article

07:10

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies

2.8K Views

article

14:56

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

18.5K Views

article

10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

10.5K Views

article

08:00

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

10.6K Views

article

09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

24.9K Views

article

09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

12.2K Views

article

09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

2.7K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone