High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
Please note that all translations are automatically generated. Click here for the English version.
2.7K Views
•
07:10 min
•
September 29th, 2023
DOI :
September 29th, 2023
•Sagar Raturi1, Huanyu Li1, Yung-Ning Chang2, Andreea Scacioc1, Tina Bohstedt1, Alejandra Fernandez-Cid1, Adam Evans1, Patrizia Abrusci1, Abilasha Balakrishnan1, Tomas C. Pascoa1, Didi He1, Gamma Chi1, Nanki Kaur Singh1, Mingda Ye1, Anna Li1, Leela Shrestha1, Dong Wang1, Eleanor P. Williams1, Nicola A. Burgess-Brown1, Katharina L. Dürr1, Vera Puetter2, Alvaro Ingles-Prieto3, David B. Sauer1
Rozdziały w tym wideo
0:00
Introduction
2:08
Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High‐Throughput Protein Expression
3:18
Purification of Human Solute Carriers (SLCs) for Developing SLC Targeting Therapies
Powiązane Filmy
12:21
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
15.0K Views
08:12
Expression and Purification of Mammalian Bestrophin Ion Channels
8.3K Views
08:27
Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
9.4K Views
08:55
Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
9.5K Views
10:32
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
17.0K Views
07:09
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
21.1K Views
07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
14.1K Views
12:16
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
24.0K Views
11:20
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
53.6K Views
07:08
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
13.7K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone