Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

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08:56 min

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June 9th, 2015

DOI :

10.3791/52795-v

June 9th, 2015

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Cyrille Ramond¹^,³, Antonio Bandeira², Claire Berthault³, Pablo Pereira³, Ana Cumano³, Odile Burlen-Defranoux³

¹Research Center Growth and Signaling, INSERM U845, Institut Cochin, ²Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, ³Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

Podsumowanie

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Keywords Thymic Settling Progenitors

T Cell Development

Lymphopenic Patients

Fetal Thymic Organ Culture

CD45 Allotypic Markers

Dendritic Epithelial T Cells

Peripheral Immune System

Thymus Grafting

CD3 Mice

Rozdziały w tym wideo

0:05

Title

1:51

Embryo Dissection

2:52

Thymus Isolation

3:49

E14 Thymic Lobe Progenitor Colonization: Hanging Drop Technique

4:53

Grafting Under the Kidney Capsule

6:22

Results: Representative Thymic Settling Progenitor (TSP) Propagation

8:07

Conclusion

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