A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
Please note that all translations are automatically generated. Click here for the English version.
11.0K Views
•
10:13 min
•
November 7th, 2015
DOI :
November 7th, 2015
•Emily J. Chadwick1, David P. Yang1, Mariella G. Filbin2, Emanuele Mazzola3, Yu Sun1, Oded Behar1,4, Maria F. Pazyra-Murphy1, Liliana Goumnerova5, Keith L. Ligon6, Charles D. Stiles1, Rosalind A. Segal1
1Department of Cancer Biology, Dana-Farber Cancer Institute, 2Department of Pediatrics, Children's Hospital, 3Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, 5Department of Neurosurgery, Children's Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Title
0:49
Dissection
2:02
Embedding the Brains in Agarose
3:28
Slicing with the Vibratome
5:31
Plating the Slices onto the Inserts
6:53
Plating the Tumor Cells and Staining
8:57
Results: Mouse Medulloblastoma Cells Treated with LDE225 (Sonidegib)
9:37
Conclusion
Powiązane Filmy
08:26
MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression
14.4K Views
07:34
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
19.3K Views
07:20
Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity
15.8K Views
08:43
Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
19.3K Views
08:26
Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
24.8K Views
12:09
A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
11.4K Views
07:48
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
20.6K Views
08:39
Three-dimensional Co-culture Model for Tumor-stromal Interaction
17.3K Views
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
20.0K Views
09:23
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
14.7K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone