Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and Metastasis
Please note that all translations are automatically generated. Click here for the English version.
2.4K Views
•
09:19 min
•
June 22nd, 2021
DOI :
June 22nd, 2021
•Rüdiger Braun1, Olha Lapshyna1, Susanne Eckelmann2,3, Kim Honselmann1, Louisa Bolm1, Meike ten Winkel1, Steffen Deichmann1, Oliver Schilling4, Charli Kruse2,3, Tobias Keck1, Ulrich Wellner1, Peter Bronsert4,5,6, Matthias Brandenburger3
1Department of Surgery, University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology, University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg, Medical Center - University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg, Medical Center - University of Freiburg
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:00
Introduction
0:54
Preparation of Low-Melting Agarose and Vibratome
1:58
Tissue Embedding in Low-Melting Agarose
3:13
Slicing of the Agarose-Embedded Tissue Using a Vibratome
3:51
Culture of Organotypic Slice Cultures
4:38
Formalin Fixation and Paraffin Embedding of OTSCs
6:20
Hematoxylin and Eosin Staining
7:09
Results: Morphology, Viability, and Histopathological Evaluation of the OTSCs at 6 and 15 Days of Primary PDAC and Metastasis
8:50
Conclusion
Powiązane Filmy
08:27
Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices
11.3K Views
10:38
An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
3.1K Views
07:48
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
20.6K Views
09:19
Culture and Imaging of Ex Vivo Organotypic Pseudomyxoma Peritonei Tumor Slices from Resected Human Tumor Specimens
1.6K Views
07:31
Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
3.7K Views
03:42
Growing Desmoplastic Three-Dimensional Pancreatic Cancer Spheroids from Co-Culture
1.3K Views
10:13
A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
11.0K Views
08:35
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
12.8K Views
07:48
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
12.8K Views
11:00
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
14.0K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone