A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

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12.6K Views

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06:53 min

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December 26th, 2015

DOI :

10.3791/53307-v

December 26th, 2015

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Hemant Suryawanshi*¹, Mayuresh Anant Sarangdhar*¹^,², Manika Vij¹^,², Reema Roshan¹, Vijay Pal Singh¹, Munia Ganguli¹, Beena Pillai¹^,²

¹CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, ²Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Podsumowanie

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Stereotactic Injection

Neurodegenerative Disease

Neuronal Cell Death

Gene Expression

Neurodevelopment

Neuronal Function

Rozdziały w tym wideo

0:00

Title

1:52

Antagomir Design Strategy

2:10

Antagomir-peptide Complex Preparation

3:08

Tail Vein Injection of Antagomir-peptide Complex

3:29

Behavioural Assays: Hind-limb Clasping

3:48

Behavioural Assays: Ledge Test

4:03

Behavioural Assays: Footprint Assay

4:32

Representative Results

6:21

Conclusion

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