Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
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16.9K Views
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10:24 min
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May 9th, 2016
DOI :
May 9th, 2016
•Brian Burkel1,2, Brett A. Morris1, Suzanne M. Ponik1, Kristin M. Riching1, Kevin W. Eliceiri2,3,4, Patricia J. Keely1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison
Rozdziały w tym wideo
0:05
Title
1:00
Polymerization of Collagen Solutions
2:23
Generation of PDMS Microchannels for Collagen Fiber Alignment
4:34
Preparation of the PDMS Microchannels and Collagen Pre-coating
6:41
Pouring Collagen Microchannels
8:26
Results: Cell Contractility and Fiber Alignment in Collagen Gels
9:26
Conclusion
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