Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

16.8K Views

•

10:24 min

•

May 9th, 2016

DOI :

10.3791/53989-v

May 9th, 2016

•

Brian Burkel¹^,², Brett A. Morris¹, Suzanne M. Ponik¹, Kristin M. Riching¹, Kevin W. Eliceiri²^,³^,⁴, Patricia J. Keely¹^,²^,⁵

¹Department of Cell and Regenerative Biology, University of Wisconsin-Madison, ²Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, ³Department of Biomedical Engineering, University of Wisconsin-Madison, ⁴Morgridge Institute for Research, University of Wisconsin-Madison, ⁵Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

Summary

Explore More Videos

Keywords 3D Collagen Gels

Chapters in this video

0:05

Title

1:00

Polymerization of Collagen Solutions

2:23

Generation of PDMS Microchannels for Collagen Fiber Alignment

4:34

Preparation of the PDMS Microchannels and Collagen Pre-coating

6:41

Pouring Collagen Microchannels

8:26

Results: Cell Contractility and Fiber Alignment in Collagen Gels

9:26

Conclusion

Related Videos

07:04

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

25.1K Views

13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

13.9K Views

08:08

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model

17.2K Views

09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

20.8K Views

09:26

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

8.4K Views

06:17

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

12.4K Views

09:03

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

13.5K Views

07:41

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

8.7K Views

10:17

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

2.1K Views

07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

2.1K Views