Generation of PDMS Microchannels for Collagen Fiber Alignment
4:34
Preparation of the PDMS Microchannels and Collagen Pre-coating
6:41
Pouring Collagen Microchannels
8:26
Results: Cell Contractility and Fiber Alignment in Collagen Gels
9:26
Conclusion
Transcribir
The overall goal of this procedure is to generate aligned and random 3D collagen matrices, for the purpose of observing cell migration or other cellular processes in the context of a 3D environment. This method is designed to capture collagen feat
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Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.