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Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

8.3K Views

11:31 min

June 30th, 2016

DOI :

10.3791/54115-v

June 30th, 2016


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High throughput

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0:05

Title

0:59

Generation of GFP Tagged c-di-GMP Reporter P. aeruginosa Strain

4:21

Preparation of the Starter Culture for Inoculation

5:35

Inoculation and Incubation of the 384-well Plates Containing Small Molecules

6:33

Measurement and Analysis of Growth and Intracellular c-di-GMP Level

8:25

Results: Hits Obtained Following the High-throughput Screen against P. aeruginosa

10:07

Conclusion

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