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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

11.4K Views

12:43 min

July 27th, 2016

DOI :

10.3791/54271-v

July 27th, 2016


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Protein Oligomerization

Rozdziały w tym wideo

0:05

Title

1:10

Y2H: Yeast Transformation

3:22

Y2H: Colony-lift Filter Paper β-Gal Assay

4:29

Protein Expression in a Mammalian Cell Line

6:56

Co-immunoprecipitation

9:02

Chemical Cross-linking

9:46

Results: The Ryanodine Receptor Forms Homo-oligomers Within Cells

11:40

Conclusion

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