Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
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12:43 min
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July 27th, 2016
DOI :
July 27th, 2016
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Rozdziały w tym wideo
0:05
Title
1:10
Y2H: Yeast Transformation
3:22
Y2H: Colony-lift Filter Paper β-Gal Assay
4:29
Protein Expression in a Mammalian Cell Line
6:56
Co-immunoprecipitation
9:02
Chemical Cross-linking
9:46
Results: The Ryanodine Receptor Forms Homo-oligomers Within Cells
11:40
Conclusion
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