Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Please note that all translations are automatically generated. Click here for the English version.

9.8K Views

•

11:04 min

•

December 24th, 2016

DOI :

10.3791/54870-v

December 24th, 2016

•

Michelle Bradley¹, Ivan Ramirez¹, Keith Cheung¹, Ankur A. Gholkar¹, Jorge Z. Torres¹^,²^,³

¹Department of Chemistry and Biochemistry, University of California, Los Angeles, ²Molecular Biology Institute, University of California, Los Angeles, ³Jonsson Comprehensive Cancer Center, University of California, Los Angeles

Podsumowanie

Przeglądaj więcej filmów

Keywords Inducible LAP tagged

Stable Cell Lines

Protein Function

Spatiotemporal Localization

Protein Interaction Networks

HEK293 Cells

Hygromycin Selection

Tandem Affinity Purification TAP

Tet Dox Induction

Anti GFP Antibody

Protein A Beads

Rozdziały w tym wideo

0:05

Title

0:44

Generation of an Inducible Stable Cell Line that Expresses the LAP-tagged Gene of Interest

2:06

Purification of LAP-tagged Protein Complexes

8:10

Identify Interacting Proteins by Mass Spectrometry Analysis

9:12

Results: Evaluation of a LAP-Tau HEK293 Inducible Cell Line

10:32

Conclusion

Powiązane Filmy

07:47

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

53.0K Views

07:57

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

16.4K Views

10:39

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

19.5K Views

09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

35.1K Views

11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

22.8K Views

11:16

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

36.9K Views

07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

17.9K Views

08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

32.6K Views

09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

12.1K Views

09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

16.9K Views