TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

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23.7K Views

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07:02 min

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May 17th, 2020

DOI :

10.3791/60728-v

May 17th, 2020

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Yongliang Zhang*¹, Yuanyuan Li*²^,³, Xinxin Yang¹, Zhiyan Wen¹, Ugrappa Nagalakshmi², Savithramma P. Dinesh-Kumar²^,³

¹State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, ²Department of Plant Biology, College of Biological Sciences, University of California, Davis, ³Genome Center, College of Biological Sciences, University of California, Davis

Podsumowanie

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TurboID

Proximity Labeling

NLR Immune Receptors

Protein protein Interactions

Nicotiana Benthamiana

Agrobacterium Tumefaciens

Agroinfiltration

Biotin Infiltration

RIPA Lysis Buffer

Supernatant Collection

Transient Interactions

Plant Defense

Rozdziały w tym wideo

0:04

Introduction

1:01

Agroinfiltration and Leaf Sample Collection

1:59

Extraction of Leaf Total Protein and Removal of Free Biotin

4:11

Enrichment of Biotinylated Proteins

5:53

Results: Western Blot of Protein Extracts

6:25

Conclusion

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