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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

9.8K Views

11:04 min

December 24th, 2016

DOI :

10.3791/54870-v

December 24th, 2016


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Inducible LAP tagged

Chapters in this video

0:05

Title

0:44

Generation of an Inducible Stable Cell Line that Expresses the LAP-tagged Gene of Interest

2:06

Purification of LAP-tagged Protein Complexes

8:10

Identify Interacting Proteins by Mass Spectrometry Analysis

9:12

Results: Evaluation of a LAP-Tau HEK293 Inducible Cell Line

10:32

Conclusion

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