Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
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15:13 min
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April 27th, 2017
DOI :
April 27th, 2017
•Tim Pieters1,2,3,4, Lieven Haenebalcke1,2, Kenneth Bruneel1,2,4, Niels Vandamme1,2,4, Tino Hochepied1,2, Jolanda van Hengel5, Dagmar Wirth6, Geert Berx1,2,4, Jody J. Haigh7, Frans van Roy1,2,4, Steven Goossens1,2,3,4
1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre
Rozdziały w tym wideo
0:05
Title
1:11
The RMCE Principle
2:15
Isolation of RMCE-compatible Knockout Mouse ES Cells
5:22
Isolate RMCE-compatible Knockout ES Cells
7:29
Generation of an RMCE-compatible Targeting Vector Using Recombination Cloning
9:25
RMCE-mediated ES Cell Targeting of Rescue Constructs to the R26 Locus and Differentiation of ES Cells in Embryoid Bodies
12:41
Results: Cystic EB Formation Using Recombinase Mediated Cassette Exchange
14:27
Conclusion
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