Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

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10.0K Views

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11:00 min

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September 18th, 2017

DOI :

10.3791/55797-v

September 18th, 2017

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Jing Gao*¹, Xiaolu Shi*², Hong He¹, Juhong Zhang¹, Ding Lin³, Guosheng Fu¹, Dongwu Lai¹

¹Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, ²Experimental Research Center, China Academy of Chinese Medical Sciences, ³Department of Cardiology, The Third Hospital of Hangzhou City

Podsumowanie

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Keywords Sarcoplasmic Reticulum

Calcium Reserve

Intracellular Diastolic Calcium Removal

Isolated Ventricular Cardiomyocytes

Langendorff Perfusion

NT Solution

Calcium free Tyrode Solution

Rozdziały w tym wideo

0:05

Title

0:44

Isolation of Left Ventricular Cardiomyocytes

4:15

Set Up of Perfusion System

4:59

Measurement of Intracellular Ca²⁺ Transient and Sarcomere Shortening

6:51

Assessment of SR Ca²⁺ Reserve and Diastolic Ca²⁺ Removal Function

9:10

Results: Assessment of SR Calcium Reserve and Calcium Removal Function

10:17

Conclusion

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