Measurement of Intracellular Ca2+ Transient and Sarcomere Shortening
6:51
Assessment of SR Ca2+ Reserve and Diastolic Ca2+ Removal Function
9:10
Results: Assessment of SR Calcium Reserve and Calcium Removal Function
10:17
Conclusion
필기록
The overall goal of this procedure is to evaluate sarcoplasmic reticulum calcium reserve and intracellular diastolic calcium removal function in isolated cardiomyocytes. This method can help answer key questions about intracellular calcium handlin
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Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.