Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Please note that all translations are automatically generated. Click here for the English version.

15.1K Views

•

11:22 min

•

February 24th, 2014

DOI :

10.3791/50898-v

February 24th, 2014

•

Ki Ho Park¹, Noah Weisleder², Jingsong Zhou³, Kristyn Gumpper¹, Xinyu Zhou¹, Pu Duann⁴, Jianjie Ma¹, Pei-Hui Lin¹

¹Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ²Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ³Department of Molecular Biophysics and Physiology, Rush University Medical Center, ⁴Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

Podsumowanie

Przeglądaj więcej filmów

Keywords Calcium Sparks

Skeletal Muscle Fibers

Ryanodine Receptor

Sarcoplasmic Reticulum

Ca2 Signaling

Osmotic Stress

Fluorescent Ca2 Indicators

Laser Scanning Confocal Microscopy

Muscle Dystrophy

Amyotrophic Lateral Sclerosis

Rozdziały w tym wideo

0:05

Title

1:29

Setting Up Osmotic-stress Perfusion System and Solution Preparation

2:00

Preparing Intact Single Flexor Digitorum Brevis (FDB) Muscle Fibers from Mouse

5:12

Fluo-4 AM Dye Loading and Ca²⁺ Imaging (Sparks Measurement)

7:39

Data Analysis

8:14

Results: Ca²⁺ Sparks in Young, Aged, and Diseased Mice

10:28

Conclusion

Powiązane Filmy

14:36

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

28.6K Views

11:10

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

24.6K Views

09:45

Mitochondrial Isolation from Skeletal Muscle

28.8K Views

12:55

Purification of Progenitors from Skeletal Muscle

18.2K Views

14:18

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

21.1K Views

14:02

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

23.7K Views

07:13

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

10.4K Views

10:25

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

18.0K Views

06:20

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

3.3K Views

07:39

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

1.2K Views