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Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

8.4K Views

10:10 min

May 28th, 2017

DOI :

10.3791/55919-v

May 28th, 2017


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Retroviral Scanning

Rozdziały w tym wideo

0:05

Title

0:45

MLV Transduction of Human Cells

1:41

Restriction Enzyme Digestion and Linker Ligation

3:05

First-round Linker-mediated PCR

3:43

Second Round Linker-mediated PCR

5:17

Library Preparation and Dilution for Massive Sequencing of MLV Integration Sites

7:53

Results: Mapping MLV Integration into Epigenetically-defined Regulatory Regions

9:23

Conclusion

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