Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

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7.9K Views

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09:38 min

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June 26th, 2019

DOI :

10.3791/59329-v

June 26th, 2019

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Jerlisa Ann C. Arizala¹^,², Pritsana Chomchan¹, Haitang Li¹, Roger Moore³, Helen Ge³, Dominique L. Ouellet¹, John J. Rossi¹^,²

¹Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, ²Irell & Manella Graduate School of Biological Sciences, ³Department of Molecular Immunology, Beckman Research Institute and the City of Hope

Podsumowanie

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HIV 1 Replication

Nucleolar Factors

Rev Immunoprecipitation

Mass Spectrometry

Viral Host Factors

HeLa Cell Line

Transfection Protocol

Lysis Buffer

Protease Inhibitor Cocktail

Molecular Immunology

Beckman Research Institute

Cell Culture Techniques

HLfB Culture Plates

Protein Characterization

Rozdziały w tym wideo

0:04

Title

0:47

Rev-nucleolar Localization Signal (NoLS)-3'Flag Mutation Expression During HIV-1 Replication

2:03

Viral Protein Lysate Collection

3:12

Rev-NoLS-3'Flag Coimmunoprecipitation

5:20

In-gel Reduction, Alkylation, and Digestion of Coomassie-stained Gel Bands

7:14

Results: Representative Nucleolar Factor Identification During HIV-1 Replication

9:05

Conclusion

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