Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
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9.1K Views
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10:22 min
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November 29th, 2017
DOI :
November 29th, 2017
•Lijuan He*1,2, Alexandra Sneider*1, Weitong Chen1, Michelle Karl1, Vishnu Prasath3, Pei-Hsun Wu1,2, Gunnar Mattson3, Denis Wirtz1,2,4
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
Rozdziały w tym wideo
0:05
Title
0:32
Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
4:00
Synchronization of Cells Stably Expressing H2B-mCherry
6:02
Incorporation of the Synchronized Cells into Collagen I Matrices
7:18
Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
8:46
Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
9:45
Conclusion
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