Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

9.1K Views

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10:22 min

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November 29th, 2017

DOI :

10.3791/56364-v

November 29th, 2017

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Lijuan He*¹^,², Alexandra Sneider*¹, Weitong Chen¹, Michelle Karl¹, Vishnu Prasath³, Pei-Hsun Wu¹^,², Gunnar Mattson³, Denis Wirtz¹^,²^,⁴

¹Department of Chemical and Biomolecular Engineering, Johns Hopkins University, ²Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, ³Department of Biomedical Engineering, Johns Hopkins University, ⁴Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

Summary

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Keywords 3D Matrices

Mammalian Cell Division

Confocal Reflection Microscopy

Cell Matrix Interactions

Cell Biology

Tissue Development

Chapters in this video

0:05

Title

0:32

Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors

4:00

Synchronization of Cells Stably Expressing H2B-mCherry

6:02

Incorporation of the Synchronized Cells into Collagen I Matrices

7:18

Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices

8:46

Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices

9:45

Conclusion

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