21.7K Views
•
11:27 min
•
August 28th, 2018
DOI :
August 28th, 2018
•Chapters in this video
0:05
Title
1:09
Preparation of CRISPR-reagents for Embryo Microinjection
4:03
Microinjection of CRISPR-components into Zebrafish Embryos
6:12
Analysis of Efficiency of Indel Formation Using an HMA
8:45
Identification and Propagation of Knock-out Lines
9:45
Results: Zebrafish Embryos Exhibit a Pigment Defect when Injected with an sgRNA Targeting Tyrosinase at the One Cell Stage
10:32
Conclusion
Related Videos
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
10.5K Views
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
12.4K Views
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
15.0K Views
Production and Purification of Baculovirus for Gene Therapy Application
14.6K Views
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
13.3K Views
CRISPR-Mediated Reorganization of Chromatin Loop Structure
12.4K Views
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
9.5K Views
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
10.1K Views
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
7.5K Views
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
7.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved