Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

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324 Views

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09:38 min

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January 24th, 2025

DOI :

10.3791/67616-v

January 24th, 2025

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Xuepu Jin*¹, Binghuang Zhang*¹, Yu Sun*¹, Yahan Duan¹, Junchen Lu¹, Jiannan Liu², Jiahuai Han¹^,³^,⁴, Yingying Zhang¹

¹State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, ²Technology Center, Shanxi Xinghuacun Fenjiu Distillery Co., Ltd., ³Research Unit of Cellular Stress of CAMS, Cancer Research Center of Xiamen University, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, ⁴Laboratory Animal Center, Xiamen University

Podsumowanie

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Genetics

CRISPR Cas9 Technology

Donor Plasmid Construction

Microinjection

Live Imaging

Protein Tracing

Physio pathological Studies

Quality Control

Troubleshooting Tips

Rozdziały w tym wideo

0:00

Introduction

1:19

Efficiency Testing of sgRNAs in Zebrafish via Microinjection and Genotyping

5:27

Donor Plasmid Construction and Microinjection for Fluorescent Protein Tagging in Zebrafish

9:01

Representative Results

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