Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

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11.2K Views

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08:08 min

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April 2nd, 2018

DOI :

10.3791/57272-v

April 2nd, 2018

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Alfonso Rodríguez-Gil¹, Tabea Riedlinger², Olesja Ritter², Vera V. Saul², M. Lienhard Schmitz²

¹Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, ²Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research, Justus-Liebig-University

Podsumowanie

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Keywords Chromatin Accessibility

Formaldehyde assisted Isolation

Regulatory Elements

Mammalian Cells

DNA protein Cross linking

Chromatin Remodeling

FAIRE

Sonication

Chromatin Fragmentation

Rozdziały w tym wideo

0:04

Title

1:03

Formaldehyde Crosslinking and Cell Harvesting

2:18

Cell Lysis and Chromatin Fragmentation

3:25

De-crosslinking of Control DNA

4:04

Phenol: Chloroform Purification of DNA

6:44

Results: Chromatin Accessibility Quantified

7:29

Conclusion

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