JoVE Logo

Zaloguj się

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

6.6K Views

11:37 min

June 18th, 2018

DOI :

10.3791/57474-v

June 18th, 2018


Przeglądaj więcej filmów

MicroRNA Transfer

Rozdziały w tym wideo

0:04

Title

0:53

CD133 SC Isolation

6:00

Characterization of Freshly Isolated CD133 SCs

7:21

Preparation of Transfection Complexes

8:26

CD133 SCs Transfection and Analysis

10:03

Results: Successful Engineering of miR-loaded Magnetized CD133 Cells

11:02

Conclusion

Powiązane Filmy

article

11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

7.1K Views

article

07:56

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

6.7K Views

article

10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

8.1K Views

article

11:16

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

7.5K Views

article

04:51

High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes

1.4K Views

article

08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

12.0K Views

article

12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

3.4K Views

article

07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

8.5K Views

article

09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

7.6K Views

article

08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

12.1K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone