Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
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Conclusion
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Our protocol can answer key questions in the field of regenerative medicine such as whether cells can be improved for the beneficial properties as well as whether they can be targeted to the site of interest. A main advantage of our protocol is th
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This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging.