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Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

12.1K Views

08:14 min

October 3rd, 2019

DOI :

10.3791/59977-v

October 3rd, 2019


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Lentiviral CRISPR

Rozdziały w tym wideo

0:04

Title

0:46

Generation and Purification of Lentivirus Particles

3:08

Isolation and Transduction of Lineage-negative Cells from Mouse Bone Marrow

5:23

Transplantation of Transduced Cells into Lethally Irradiated Mice

5:39

Evaluating the Chimerism of Peripheral Blood

6:19

Results: Lentiviral Transduction of Mouse Bone Marrow Lineage-negative Cells

7:23

Conclusion

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