Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

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10.5K Views

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09:31 min

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March 7th, 2019

DOI :

10.3791/57645-v

March 7th, 2019

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Masaya Ikegawa*¹, Takashi Nirasawa*², Nobuto Kakuda¹, Tomohiro Miyasaka¹, Yuki Kuzuhara¹, Shigeo Murayama³, Yasuo Ihara⁴

¹Department of Life and Medical Systems, Doshisha University, ²Bruker Daltonics K.K., ³The Brain Bank for Aging Research, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, ⁴Graduate School of Brain Science, Doshisha University

Podsumowanie

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Keywords Amyloid Beta

MALDI Imaging Mass Spectrometry

Subarachnoid Vasculature

Cryostat

ITO coated Glass Slides

Ethanol Wash

Vacuum Drying

Rozdziały w tym wideo

0:04

Title

0:51

Preparation of Tissue Sections for Imaging Mass Spectrometry (IMS)

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MALDI-IMS

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Results: Visualization of Aβ and Its Isoforms from Autopsied Brains with AD and CAA with MALDI-IMS

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Conclusion

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