JoVE Logo

Sign In

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

11.0K Views

09:45 min

August 18th, 2018

DOI :

10.3791/58223-v

August 18th, 2018


Explore More Videos

Oligonucleotide

Chapters in this video

0:04

Title

0:32

Antisense Oligonucleotide (ASO) Design

2:22

Protein Extract Preparation

4:08

Step 1: Poly(A) RNA Isolation

5:45

Step 2: Specific mRNA Capture with 3 -Biotinylated 2 -Methoxy Modified Antisense RNA Oligonucleotides

7:41

Results: Capture of Specific mRNA-protein Complexes from Extracts Derived from UV Crosslinked Cells with TRIP

9:00

Conclusion

Related Videos

article

10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

9.0K Views

article

11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

8.9K Views

article

11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

14.9K Views

article

09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

25.1K Views

article

10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

9.6K Views

article

08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

7.2K Views

article

13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

11.7K Views

article

11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

19.8K Views

article

13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

27.5K Views

article

14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

12.8K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved