Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Please note that all translations are automatically generated. Click here for the English version.
6.7K Views
•
09:07 min
•
May 1st, 2019
DOI :
May 1st, 2019
•Przeglądaj więcej filmów
Rozdziały w tym wideo
0:04
Title
0:53
Inoculum Preparation
1:34
Growth and Harvesting of Mycelia
2:36
Preparation of the Protein Extract and Equilibration of the IgG Resin
4:40
Batch Purification of TAP-tagged HDAC
6:50
Results: Analysis of the Purification and Enzymatic Activity Assay
8:29
Conclusion
Powiązane Filmy
09:52
A Fluorescence-based Assay of Phospholipid Scramblase Activity
13.8K Views
14:32
Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
8.2K Views
09:20
Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
8.4K Views
11:36
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
10.6K Views
09:11
Assays for Validating Histone Acetyltransferase Inhibitors
6.4K Views
07:26
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
3.9K Views
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
983 Views
05:44
An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
734 Views
09:43
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
21.2K Views
12:07
Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
13.9K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone