In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Please note that all translations are AI generated. Click here for the English version.

10.6K Views

•

11:36 min

•

July 25th, 2019

DOI :

10.3791/59385-v

July 25th, 2019

•

Louis Masclef*¹, Uriarte Maxime*¹, Oumaima Ahmed¹, Nadine Sen Nkwe¹, Haithem Barbour¹, Nicholas V.G. Iannantuono¹, Amina Boubekeur¹, Salima Daou¹^,², El Bachir Affar¹^,²

¹Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, ²Lunenfeld-Tanenbaum Research Institute, Sinai Health System

Podsumowanie

Przeglądaj więcej filmów

Small Molecule Inhibitors

High Throughput Screening

Rozdziały w tym wideo

0:04

Title

1:01

GSH-agarose Affinity Purification of the GST-RING1B(1-159)-BMI1 (1-109) E3 Ubiquitin Ligase Complex

2:16

Purification of the BAP1/DEUBAD (ASXL2) Deubiquitinase Complex

4:58

Purification of the Nucleosomes from HEK293T Cells

7:38

Nucleosome Ubiquitination Assay Using BMI1/RING1B E3 Ubiquitin Ligase Complex and In Vitro Nucleosomal H2A DUB Assay Using BAP1/DEUBAD

9:05

Results: Purification, Ubiquitination, and Deubiquitination

10:40

Conclusion

Powiązane Filmy

08:06

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

7.6K Views

09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

10.5K Views

08:37

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

14.0K Views

09:35

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

6.9K Views

06:08

Quantification of Hypopigmentation Activity In Vitro

11.1K Views

12:25

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

10.2K Views

11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

9.3K Views

07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

3.9K Views

08:57

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

5.7K Views

08:16

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

2.6K Views