Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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7.3K Views

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10:06 min

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July 10th, 2019

DOI :

10.3791/59609-v

July 10th, 2019

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Jiahuan Chen¹, Ying Xu², Yan Shi², Tie Xia²

¹State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, ²Institute for Immunology, School of Medicine, Tsinghua University

Podsumowanie

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Atomic Force Microscope

Cantilevers

Single T Cells

Biocompatible Glue

Immune Cell Activation

Intercellular Interactions

Dendritic Cell T Cell Interactions

Rozdziały w tym wideo

0:04

Title

1:17

AFM Cantilever Preparation

2:36

Attaching Single T-cells to the Cantilever

4:58

Force Spectroscopy of Single-pair T-cell/Dendritic Cell Interaction

5:34

Cantilever Functionalization with Single Polystyrene Beads

7:44

Results: Measuring Cell-Cell Adhesions

9:30

Conclusion

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