In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
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8.8K Views
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09:19 min
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May 24th, 2020
DOI :
May 24th, 2020
•Xue D. Manz1, Hugo J. Albers2,3, Petr Symersky4, Jurjan Aman1, Andries D. van der Meer3, Harm Jan Bogaard1, Robert Szulcek1,5,6
1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center
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Rozdziały w tym wideo
0:04
Introduction
0:59
Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
2:43
Flow Chambers and PAEC Monolayer Preparation
4:00
Washed Human Whole Blood Preparation
4:50
Flow System Assembly
5:41
Washed Whole Blood Perfusion
6:44
Results: Representative Thrombus Formation Characterization
8:29
Conclusion
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