Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

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5.7K Views

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14:02 min

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October 31st, 2020

DOI :

10.3791/61434-v

October 31st, 2020

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Luca Russo¹, Carmina Natale¹, Andrea Conz¹, Joe Kelk², Elena Restelli², Roberto Chiesa², Mario Salmona¹, Luana Fioriti²^,³, Luca Colnaghi¹

¹Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, ²Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, ³Dulbecco Telethon Institute, Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Podsumowanie

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Super resolution Imaging

Co localization

Primary Neurons

Synaptic Markers

Structured Illumination Microscopy

Imaging Techniques

Neurodegenerative Disorders

Statistical Methods

Hippocampal Neurons

Paraformaldehyde Fixation

Antibody Background Reduction

BSA Blocking Buffer

SIM compatible Mountant

Coverglass Mounting

Rozdziały w tym wideo

0:00

Introduction

1:04

Primary Cultures

1:48

Immunofluorescence Staining

3:32

SIM Microscopy

4:01

Microscope Calibration

7:11

Acquisition

9:24

Post-production: Image Reconstruction

11:24

Colocalization with Profile Analysis

12:05

Quantification of Pearson's and Mander's

12:27

Results

13:16

Conclusions

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