Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

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6.3K Views

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07:25 min

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June 25th, 2020

DOI :

10.3791/61478-v

June 25th, 2020

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Owen Hudson¹, Sumyya Waliullah¹, Justin Hand², Romina Gazis-Seregina³, Fulya Baysal-Gurel⁴, Md Emran Ali¹

¹Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, ²University of Georgia Cooperative Extension, ³Department of Plant Pathology, Tropical Research & Education Center, University of Florida, ⁴Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

Podsumowanie

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Phytophthora Capsici

Irrigation Water

Detection

Loop mediated Isothermal Amplification

Plant Disease Management

Rozdziały w tym wideo

0:04

Introduction

0:48

Pump and Filter Setup

1:16

Water Sampling and Filtering

2:22

Magnetic Bead-Based DNA Extraction

3:58

Loop-Mediated Isothermal Amplification (LAMP) Assay

5:07

Results: Representative Phytophthora capsici Detection

6:53

Conclusion

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