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JoVE Journal

Environment

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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

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07:25 min

June 25th, 2020

June 25th, 2020

6,280 Views

0:04

Introduction

0:48

Pump and Filter Setup

1:16

Water Sampling and Filtering

2:22

Magnetic Bead-Based DNA Extraction

3:58

Loop-Mediated Isothermal Amplification (LAMP) Assay

5:07

Results: Representative Phytophthora capsici Detection

6:53

Conclusion

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Phytopathogen detection is a crucial step in plant disease management. This protocol provides a rapid detection method for irrigation water contamination due to a common waterborne fighter pathogen. Using this technique, specific pathogen, such as

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We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.

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