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Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

2.9K Views

11:04 min

September 3rd, 2020

DOI :

10.3791/61493-v

September 3rd, 2020


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Rat Brain

Rozdziały w tym wideo

0:04

Introduction

0:47

Construction of Bilateral Cannulas, Obturators, and Caps

2:49

Construction of Microinjectors

4:10

Animal Maintenance and Vaginal Smears

5:31

Microinjection of TTX or Vehicle Solutions into Freely Moving Rats

7:27

Tissue Processing

8:44

Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)

10:08

Conclusion

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