Sign In

JoVE Journal

Neuroscience

A subscription to JoVE is required to view this content.

Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Transcript

This protocol allows researchers to explore the nuclei of the neuronal circuits that regulates ovulation in female mammals at specific times of their estrous cycle.This technique can be adapted to study any process on the regulation of the brain i

Sign in or start your free trial to access this content

This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More