Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

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3.4K Views

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06:42 min

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September 1st, 2020

DOI :

10.3791/61733-v

September 1st, 2020

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Rupam Ghosh*¹, Jaka Kragelj*¹, Yiling Xiao*¹, Kendra K. Frederick¹^,²

¹Department of Biophysics, University of Texas Southwestern Medical Center, ²Center for Alzheimer's and Neurodegenerative Disease and Center for Systems Biology, University of Texas Southwestern Medical Center

Podsumowanie

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Cryogenic Sample Loading

Magic Angle Spinning

Nuclear Magnetic Resonance

DNP Assisted MAS NMR

Cellular Viability

Sample Handling

Cold Rotor Introduction

Moisture Build Up

Cryogenic Transfer Protocol

Rozdziały w tym wideo

0:04

Introduction

0:49

Cryopreservation of Mammalian Cells in an NMR Rotor

1:25

Cryogenic Transfer of a Frozen Sample into the NMR Spectrometer

3:48

Cryogenic Removal of the Sample from the NMR Spectrometer

5:02

Results: Cryogenic Insertion of a Pre-frozen Sample of Mammalian Cells Results in Cellular Intactness Similar to Never-frozen Mammalian Cell Samples

5:47

Conclusion

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