Location, Dissection, and Analysis of the Murine Stellate Ganglion

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9.8K Views

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07:50 min

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December 22nd, 2020

DOI :

10.3791/62026-v

December 22nd, 2020

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Katharina Scherschel¹^,²^,³, Hanna Bräuninger²^,⁴, Klara Glufke², Christiane Jungen²^,⁴^,⁵, Nikolaj Klöcker³, Christian Meyer¹^,²^,³

¹Division of Cardiology, EVK Düsseldorf, cNEP, cardiac Neuro- and Electrophysiology Research Consortium, ²DZHK (German Centre for Cardiovascular Research), ³Institute of Neural and Sensory Physiology, Medical Faculty, Heinrich Heine University Düsseldorf, ⁴Clinic for Cardiology, University Heart & Vascular Centre, University Hospital Hamburg-Eppendorf, ⁵Department of Cardiology, Leiden University Medical Center

Podsumowanie

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Murine Stellate Ganglion

Sympathetic Nervous System

Thoracotomy Procedure

Sudan Black

Autofluorescence Reduction

Antibody Permeabilization

Preparation Techniques

Rozdziały w tym wideo

0:04

Introduction

1:06

Location and Dissection of Murine Stellate Ganglia

2:01

Whole Mount Immunohistochemistry Protocol

5:23

Imaging and Analyses of Murine Stellate Ganglia

5:44

Results: Qualitative and Quantitative Analysis of Murine Stellate Ganglia

7:14

Conclusion

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