Location and Dissection of Murine Stellate Ganglia
2:01
Whole Mount Immunohistochemistry Protocol
5:23
Imaging and Analyses of Murine Stellate Ganglia
5:44
Results: Qualitative and Quantitative Analysis of Murine Stellate Ganglia
7:14
Conclusion
Trascrizione
Every heartbeat is under tight control of the sympathetic nervous system. Most neurons within cardiac neurocontrol interact with the stellate ganglia. Our protocol makes it possible to study the underlying processes.
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Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.