Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

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1.7K Views

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07:17 min

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April 26th, 2021

DOI :

10.3791/62352-v

April 26th, 2021

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Janet Y. Sheung¹, Megan Otsuka², Gabriella Seifert³, Athena Lin⁴, Wallace F. Marshall⁴

¹W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, ²W. M. Keck Science Department, Pitzer College, ³W. M. Keck Science Department, Scripps College, ⁴Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

Podsumowanie

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Stentor Coeruleus

Motility Patterns

Unicellular Regeneration

Oral Apparatus Regeneration

Cell Tracking Assay

Imaging Chamber Construction

Silicone Spacer Sheets

Time Lapse Video

Microscope Calibration

Data Organization

Sample Preparation

Regeneration Timeline

Functional Regeneration

Video Analysis

Rozdziały w tym wideo

0:05

Introduction

1:03

Sample Preparation

2:15

Visible Light Microscopy Time-Lapse

2:56

Cell Tracking

3:52

Trace Verification

4:26

Data Visualization

5:14

Results: Motility Patterns of Stentor During and After Oral Apparatus

6:34

Conclusion

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