JoVE Logo

Zaloguj się

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

7.9K Views

00:08 min

August 27th, 2019

DOI :

10.3791/59995-v

August 27th, 2019


Przeglądaj więcej filmów

Single Cell Durotaxis Assay

Rozdziały w tym wideo

0:04

Title

0:41

Glass Coverslip Activation

1:15

Fluorescent Microbead Deposition and Hydrogel Casting

2:38

Hydrogel Activation and Extracellular Matrix Coating

3:38

Cell Plating and Micropipette Preparation

5:51

Micromanipulator Calibration and Durotaxis Assay

7:01

Results: Representative Durotactic Stimulation Responses and Protein Expression Analyses

7:55

Conclusion

Powiązane Filmy

article

10:23

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

9.8K Views

article

07:15

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

7.4K Views

article

11:00

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

843 Views

article

09:20

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

11.2K Views

article

09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

4.7K Views

article

11:37

Measurement of Cellular Chemotaxis with ECIS/Taxis

14.7K Views

article

09:50

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

8.2K Views

article

10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

6.9K Views

article

Protocol for Analysis and Consolidation of TrackMate Outputs for Measuring Two-Dimensional Cell Motility using Nuclear Tracking

1.2K Views

article

14:55

Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

3.9K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone