Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
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1.5K Views
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05:50 min
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January 20th, 2022
DOI :
January 20th, 2022
•Alexandre Dias Tavares Costa1,2,3, Steffanie Skau Amadei1,3, Amanda Bertão-Santos1,2, Tuany Rodrigues1,3
1Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), 2Pós-graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná (UFPR), 3Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz)
Rozdziały w tym wideo
0:04
Introduction
0:47
Preparation of Stock Solutions and Gelification Mixture
2:53
Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR
4:22
Results: Ready to Use Gelified qPCR for Trypanosoma cruzi Epimastigotes DNA Detection
5:11
Conclusion
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