Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
1.4K Views
•
05:50 min
•
January 20th, 2022
DOI :
January 20th, 2022
•Alexandre Dias Tavares Costa1,2,3, Steffanie Skau Amadei1,3, Amanda Bertão-Santos1,2, Tuany Rodrigues1,3
1Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), 2Pós-graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná (UFPR), 3Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz)
Chapters in this video
0:04
Introduction
0:47
Preparation of Stock Solutions and Gelification Mixture
2:53
Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR
4:22
Results: Ready to Use Gelified qPCR for Trypanosoma cruzi Epimastigotes DNA Detection
5:11
Conclusion
Related Videos
06:18
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
14.2K Views
09:23
Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
13.8K Views
06:13
Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
8.9K Views
08:48
In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
3.2K Views
13:55
Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
15.1K Views
08:21
Chemical Cartography Approaches to Study Trypanosomatid Infection
2.2K Views
08:17
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
3.0K Views
07:59
Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies
1.3K Views
08:37
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
13.5K Views
09:45
Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi
724 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved