Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

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1.4K Views

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05:50 min

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January 20th, 2022

DOI :

10.3791/63316-v

January 20th, 2022

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Alexandre Dias Tavares Costa¹^,²^,³, Steffanie Skau Amadei¹^,³, Amanda Bertão-Santos¹^,², Tuany Rodrigues¹^,³

¹Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), ²Pós-graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná (UFPR), ³Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz)

Podsumowanie

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QPCR

Trypanosoma Cruzi

Pathogenic Organisms

Gelification Protocol

Operator Error Prevention

Visual Demonstration

Rozdziały w tym wideo

0:04

Introduction

0:47

Preparation of Stock Solutions and Gelification Mixture

2:53

Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR

4:22

Results: Ready to Use Gelified qPCR for Trypanosoma cruzi Epimastigotes DNA Detection

5:11

Conclusion

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